This powerful tool enables comparisons of Richness between samples differing in size. Richness is the number of unique TCR sequences in a sample and is one of the two quantitative components of diversity. Since most immunosequencing data sets are strongly dominated by low-frequency unique rearrangements, sample size is the principle determinant of Richness. By downsampling each sample to a pre-defined number of cells (productive templates), this key component of diversity can be compared between different sized samples.

This tool works by randomly selecting a designated number of productive templates and calculating metrics such as Richness and Clonality. The process is repeated, and the results averaged to generate downsampled values for each sample.

The new Downsampled Metrics tool is available in the Tools Menu in the immunoSEQ Analyzer. For more information select the "Learn more" link in the tool introduction or contact our Technical Support team at techsupport@adaptivebiotech.com